Coding

Part:BBa_K5165000:Design

Designed by: Ziyang Yin   Group: iGEM24_Concordia-Shanghai   (2024-09-29)


Polyhydroxybutyrate (PHB) depolymerase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 616
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 553
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 581


Design Notes

No particular design considerations were taken as the sequence was mostly taken from UniProt. The Talaromyces funiculosus organism's PHB depolymerase was chosen as it only depolymerized PHB into the monomer BHB and no dimers or trimers would be formed. Additionally, the Talaromyces funiculosus's PHB depolymerase functions in the extracellular space, and hence would not require cell lysis for the extraction of BHB.


Source

ATGTTCGACTCGGTTAAGATTGCATGGCTCGTGGCACTTGGTGCTGCTCAAGTCGCAGCGACGGCCCTACCTGCCTTCAATGTCAATCCAAACTCTGTTTCTGTCTCTGGTCTATCGAGCGGTGGATATATGGCAGCTCAGCTTGGAGTTGCGTACTCTGACGTTTTCAACGTTGGATTCGGCGTCTTTGCTGGTGGGCCTTATGATTGTGCTCGTAACCAATACTATACTTCGTGCATGTATAATGGCTATCCTTCTATTACCACTCCCACCGCCAATATGAAATCCTGGAGTGGAAATCAGATTGCCAGCGTGGCAAATCTCGGGCAACGAAAGATTTACATGTGGACTGGATCTTCCGACACTACAGTCGGACCAAACGTCATGAACCAGTTAAAGGCACAGTTGGGCAACTTTGACAACTCGGCCAATGTCTCGTACGTTACAACAACTGGTGCCGTTCACACATTTCCCACCGATTTCAATGGTGCCGGAGATAACTCTTGTAGCCTTTCCACCTCCCCATATATTAGCAACTGCAACTATGATGGTGCCGGCGCCGCTCTCAAATGGATTTATGGGTCTCTAAATGCAAGAAATACAGGCACTTTGTCGGGATCCGTCCTCTCCTTTGCCCAGTCGGGCAGCTATGGAGCTAACGGTATGGACACCACGGGTTATCTGTACGTGCCTCAGAGCTGTGCAAGCGGTGCGACAGTTTGTTCTTTGCATGTCGCCTTGCATGGGTGTCTGCAAAGCTATAGCTCTATTGGTTCGAGATTTATACAAAACACTGGATACAACAAATGGGCAGACACAAACAACATGATTATCCTGTATCCTCAAGCCATCCCCGACTATACTATTCATGCAATTTGGAATGGAGGGGTGCTTTCTAACCCCAATGGTTGCTGGGATTGGGTAGGCTGGTATGGAAGTAATGCTGATCAGATTGGTGGCGTTCAAATGGCTGCTATTGTTGGCCAGGTCAAGCAGATTGTCAGTGGCTTTCAAGGCTAG

References